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he success of nanoscale formats for LC-MS has been driven primarily by the need to analyze lowconcentration samples and/or samples where quantities are limited, yet the need for higher sensitivity and higher throughput continues to grow.Miniaturization of the ESI LC-MS format has become the standard for proteomic analysis. This format provides a powerful approach to manipulate and deliver small quantities of sample with significant improvements in sensitivity. Here we demonstrate the novel application of nanobore LC-MS to the analysis of a spiked standard in plasma using a protein precipitation sample preparation protocol. Variable-flow LC-MS “peak parking” is applied to improve nanospray response without negatively impacting analysis time.

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